What is the infected file name, where was it found e.g. (C:\windows\system32\infected-file-name.xxx) ?
Check the avast! Log Viewer (C:\Program Files\Alwil Software\Avast4\ashLogV.exe
), Warning section, this contains information on all avast detections.
If you have XP, vista32bit or Win2k, you could enable a boot time scan. Right click the avast icon, select Start avast! Antivirus, a memory scan will take place followed by the opening of the Simple User Interface, Menu, 'Schedule boot-time scan...' Or see
http://www.digitalred.com/avast-boot-time.php.
That should get round any file in use message as the scan happens outside windows so the file shouldn't be in use. When detected in the boot-time scan do the same, send to the chest.