Whats the point of detecting something extinct?? When if you do run then on today's MS supported OS like Win7/Win8 those guys wouldnt run on them or they would run and then be unable to do anything and crash.
Those MS-DOS stuff are dinosaurs in the malware age.What's the point of wasting time and energy on detecting something that is not going to be alive in the real-world for not more than a day or less or which is years old like MS-DOS viruses.