I don't know where you read it is safe to delete.
But I would say the short answer is you can't, it is protected by avasts Self Defence module, like files in the avast folder locations.
Not to mention if it is related the virus definitions, even if you were able to defragment it, that could corrupt it. Virus definitions are constantly being updated so there is going to be change (but 1400 seems excessive.
That said I don't have that file in that folder, see attached image 1.
The main area for virus definitions is, C:\Program Files\AVAST Software\Avast\defs, see image 2
Mainly I would say, even if this file were to be able to defragment this file there is no guarantee it would reduce the size very much.