I honestly don't know, but if it did, I certainly wouldn't consider it as there can be some huge files, your swap file for instance and you wouldn't want to send that to the chest.
The current default max file size to send is 16MB (in KBs), that is I feel a reasonable size, if there does ever happen to be something larger it would display an error and you can choose whether to increase the size to allow it to be sent.
All of this would have to be related to, what are you going to do with it once in the chest. If you wanted to send it for analysis you would find that there is an upload limit there also (I don't know what that is as I haven't encountered it, but there is one).
The other thing is that malware files I would say aren't going to be massive, so in general they should be under the existing 16MB default size limit.